Bovine hepatocyte growth factor and its receptor c-Met: cDNA cloning and expression analysis in the mammary gland
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概要
- 論文の詳細を見る
Hepatocyte growth factor/scatter factor (HGF/SF) is a pleiotropic cytokine that plays a crucial role in the embryonic and postnatal development of various organs including the mammary gland. We cloned bovine HGF and its c-Met receptor cDNAs, and examined their expression during mammary gland development in dairy cows. The 2.5-kbp HGF cDNA clone contained a 2,190 bp open reading frame coding a 730 amino acid protein, while the 4.8-kbp c-Met cDNA clone contained a 4,152 bp open reading frame coding a 1,384 amino acid protein. The bovine HGF and c-Met sequences exhibited more than 87% identity with those of other mammals. RT-PCR analysis revealed ubiquitous expression of both HGF and c-Met mRNAs in various bovine tissues tested. HGF mRNA was detected only in the inactive stage of bovine mammary gland development and not in the developing, lactating, and involuting stages, while c-Met mRNA was detected in the inactive and involuting stages. Immunohistochemical analysis demonstrated that the c-Met protein was found on mammary epithelial cells in the inactive, developing, and involuting stages, and on myoepithelial cells in all stages. These results suggest pivotal roles of HGF and c-Met in the development of bovine mammary gland.http://www.sciencedirect.com/science/journal/07397240
- Elsevierの論文
著者
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アハメド モハメド
北海道大学大学院獣医学研究科生化学教室
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Saito Masayuki
日本獣医生命科学大学 獣医保健看護学科臨床部門
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Saito Masayuki
Department Of Nutrition Graduate School Of Nursing And Nutrition Tenshi College
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Kimura K
Laboratory Of Biochemistry Department Of Biomedical Sciences Graduate School Of Veterinary Medicine
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