犬糸状虫仔虫の走査電子顕微鏡的観察

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走査型電子顕微鏡を用いて犬糸状虫Dirofilaria immitis仔虫の体表微細構造を観察した.仔虫の体表には頭端より尾部まで虫体長軸に直角に,ほぼ等間隔に走る約310個の輪状溝が存在する.各体環表面は全く平滑である.時に輪状溝の分岐が見られる.頭端は半球状を呈し,その一端より長さ0.4～0.6μの鉤が後方にのぴる.又頭端にはCephalic ciliary channelとBuccal capsuleの開口部と考えられる2つの小孔が,頭端中央部と鉤の対側に存在する.排泄孔は頭端より約83番目の体環中央部に開き,一般に円形を呈す.肛門孔は尾端よりほぼ93番目の体環中央部に存し排泄孔より大きく楕円形を呈している.尾端に近づくにつれ,各体環は小さくなり,尾端近くでは一見連珠様を示す.尾端より4-5μの部分は棍棒状でその表面には輪状溝は認められない.The microfilariae separated from the blood were washed three times in phosphate buffer solution adjusted to pH 7.2, fixed in 5% glutaraldehyde, dehydrated in a graded series of aceton. Under scanning electron microscope, one can clearly observe the striated annular appearance running across transversely with regular intervals on the surface of cuticle all over the body. In high power micrograph, the body surface is almost smooth and pits, tubercles and any other attachments are not recognizable. The number of the annulation is approximately 310. The anterior tip of microfilaria forms a cap-llike cephalic disk which is provided with a single wedge-shaped hook and two small pores. The hook measures about 0.4-0.6μ in length by 0.3μ in width at the base. The pores are round in shape measuring about 0.1μ in diameter, one of which opens at the center of cephalic disk and another one at the lateral side opposite to the hook. These two pores may be the orifices of buccal capsule and cephalic ciliary channels. The excretory pore is a small round opening, which is situated at nearly the 83rd annulus ranked from the anterior tip. The oval-shaped anal pore is usually demonstrated at around the 93rd annulus ranked from the posterior end. The size is generally larger than the excretory pore, measuring 0.4-0.5μ in diameter. The posterior extremity gradually tapers and is finally connected to the club-shaped tail. The tail is about 4-5μ long lacking the transverse striations on the surface.
長崎大学熱帯医学研究所,Institute of Tropical Medicine, Nagasaki Universityの論文
1975-03-25

犬糸状虫仔虫の走査電子顕微鏡的観察

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