植物細胞壁崩壊酵素を生産する糸状菌の検索

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Thirty strains of Amylomyces, Fusarium, and Rhizopus were screened for the production of plant cell wall-degrading (PCWD) enzymes when they were cultured on medium containing wheat bran only. A. rouxii MIBA360 produced the highest quantities of PCWD enzyme, which was closely correlated with the quantity of pectinase produced. On the other hand, F. solani f. sp. asparagi MIB366 produced the highest amount of cellulase, although all the Fusarium species tested did not produce PCWD enzyme. The PCWD enzyme was most active at 55℃ and pH 5.0. The enzyme was stable after heating it for 1h at pH 6.0 and at 37℃. Full enzyme activity was maintained after heating for 16h between pH 6.0 and 7.5. After incubating with plant tissues such as bean curd lees, Japanese radish, Chinese cabbage, Japanese green (Mibuna) and potato, at 37℃ for 4 days, the PCWD enzyme preparation degraded 60-80% of the plant tissues.
2013-12-24