Multiplication of Pinellia ternata by Callus Culture of Leaf Segment

概要

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Callus culture of Pinellia ternata has been established in which the leaf obtained from the tissue culture of tuber segment was inoculated onto Murashige and Skoog's (MS) medium supplemented with 2 ppm 2,4-dichlorophenoxyacetic acid (2,4-D) and 1-2 ppm kinetin. Regeneration from callus segment was stimulated in a medium containing 1 ppm a-naphthaleneacetic acid (NAA) to produce 50.7 plantlets per callus segment. The callus segment could be sub-cultured to multiply callus retaining its regeneration potential. As regards the effects of low temperature storage at 4℃ and 0℃ on callus growth rate and regeneration capacity, the storage of callus segment at 4℃ was found to increase the propagation capacity of callus and to its regeneration capacity compared to the control. When the callus segment was stored at 0℃, the regeneration potential and the multiplication capacity of callus decreased and finally disappeared after 20 weeks.
日本生薬学会の論文
1986-06-20