In Vitro Propagation of Epimedium Species

概要

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Shoot tip of Epimedium diphyllum (MORR. et Decne.) Lodd. (baika-ikariso in Japanese) were cultured on Murashige and Skoog (MS) medium supplemented with 4.4 μM 6-benzylaminopurine (BAP) and 2.9 μM gibberellic acid A_3 (GA) or on MS medium without growth regulators to form shoots. The regenerated shoots formed roots on the growth regulator-free MS medium after four subcultures. Organogenesis from immature seeds of E. diphyllum occurred most efficiently when cultured on MS medium containing 1.1 μM 2,4-dichlorophenoxy acetic acid (2,4-D) and 1.1 μM BAP to give an 80-fold propagation after 2-month culture. For regeneration, MS medium containing 0.73-1.45 μM GA and 0.44-2.2 μM BAP was the most suitable. After four subcultures on growth regulator-free MS medium, these regenerated shoots formed plantlet. After 6 month of culture of immature seeds of E. grandiflorum MORR. var. higoense T. SHIMIZU (higo-ikariso in Japanese) on the MS medium supplemented with 4.5 μM 2,4-D callus induction occurred without organogenesis. The medium containing 1.13 μM 2,4-D and 4.4 μM BAP was the best for callus propagation. A half- strength MS medium containing 4.5 μM or 2.25 μM 2,4-D and 1% sucrose stimulated organogenesis. A half-strength MS medium containing 1.45 μM GA and 0.44 μM BAP or 1/4 strength MS medium supplemented with 1.45 μM GA and 2.2 μM BAP was used to promote organogenesis.
日本生薬学会の論文
1994-03-20