イオン電極法による発酵TMRおよびサイレージ中のアンモニア態窒素濃度の簡易測定

概要

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The relationship between data on volatile basic nitrogen (VBN) content in fermented TMR and silage samples measured by microdiffusion analysis and ion electrode method was examined in order to develop its more simplified measurement than conventional ones. A total of 68 fermented TMR and silage samples including fodder rice, sugar cane, rice shochu distillery byproduct etc. were used for simple regression analysis. From the results of comparison of 30, 50 and 100 mL samples of fermented TMR ex- tracts, it was found that 30 mL was enough volume for determination by ion electrode method. A significant linear regression was obtained between data on VBN content by microdiffusion analysis (Y) and ion electrode method (X), i.e. Y=0.955X (r=0.980, P<0.001). Therefore, it was suggested that ion electrode method was applicable to determination of VBN content using 30mL extracts.
2012-01-15