和漢薬の基源に関する研究 : 組織形態学,ケモタキソノミー,遺伝子解析の応用

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Herbal drugs are quite variable in the quality due to their natural and artificial conditions. This variation affects in turn, the quality and efficacy of the pharmaceutical preparations, including those crude drugs, and thus may cause problems in their therapeutic applications. Therefore, the quality evaluation of herbal drugs has always been a very serious problem. For the quality evaluation we have focused on the authentication of herbal drugs through 1) comparative anatomical study, 2) chemotaxonomical study, and recently, 3) gene analysis. In the present review, studies on "Shengma," citrus drugs and Ginseng drugs by the above three approaches, respectively, are described. 1) A key standard for identification of the botanical origins of "Shengma" was established on the basis of the anatomical characteristics of the underground parts of the plants of the genera Cimicifuga, Astilbe, Aruncus, Strobilanthes and Serratula. The market samples were identified on the basis of this key standard. 2) HPLC analyses of 34 marker compounds including polymethoxyflavonoids, coumarins and flavonoid-glycosides were performed on the fruit peels of the plants of the genera Citrus, Fortunella and Poncirus. According to the HPLC profiles, the citrus peels were divided into 14 types, and the origins of citrus drugs were identified on the basis of their chemical composition. 3) The 18S ribosomal RNA genes of Panax ginseng, P. japonicus and P. quinquefolius had species-specific sequences, and the sequence analysis revealed the origins of their derivative drugs. An ultimate identification method of Ginseng drugs by the PCR-RFLP and ARMS methods was established.
日本生薬学会の論文
2001-04-20

和漢薬の基源に関する研究 : 組織形態学,ケモタキソノミー,遺伝子解析の応用

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