P-25 モウソチクキシランのキシリトール発酵(ポスターセッション2:技術04,研究発表(ポスター発表))

概要

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To prepare a substrate for microbial conversion of xylose into xylitol, the culm of Phyllostachy heterocycla was hydrolyzed with 3% sulfuric acid at 121℃ for 1 h. A fermentable substrate with a relatively high xylose concentration (61.1 g L^<-1>) was obtained by hydrolysis with 3% sulfuric acid with a liquid to solid ratio of 3 g g^<-1>. During hydrolysis at elevated temperatures, certain undesired byproducts were also generated, such as degradation products of solubilized sugars and lignin, which are potential inhibitors of microbial metabolism. These compounds were, however, successfully removed from the hydrolyzate by treatment with a commercial steam-activated char. Microbial conversion of xylose in the detoxified hydrolyzate into xylitol was carried out using a yeast Candida magnoliae. Xylitol could be obtained by the fermentation in a 70% yield. However, a reduction in fermentation time would be needed.
2011-01-12