P-26 シラカンバキシランからキシリトール発酵生産(糖化・マテリアル,Poster Session)

概要

論文の詳細を見る
A sugar solution containing 43 g L^<-1> xylose was prepared from the wood of Japanese white birch by hydrolysis with 3% sulfuric acid with a liquid-to-solid ratio of 4 g g^<-1> at 120℃ for 1 h. During the acid hydrolysis, some byproducts were also generated, such as acetic acid, furfural, 5-methylhydrofurfural, and low molecular phenolics. These compounds inhibit the conversion of xylose to xylitol. When the hydrolyzate was treated by steam-activated charcoal (15 g L^<-1>), these inhibitors, except acetic acid, were successfully removed from the hydrolyzate with no significant changes in sugar concentrations. The hydrolyzate was then treated by an anion exchange resin (80 g L^<-1>) for 1 h to remove acetic acid. Bioconversion of the detoxified hydrolyzate to xylitol using the yeast, Candida magnoliae was investigated under strictly controlled oxygen supply condition. The oxygen transfer rate (OTR) varied from 10.0 to 20.0 mmol-O_2 L^<-1> h^<-1>. Under the best OTR of 15.0 mmol-O_2 L^<-1> h^<-1>, a solution containing 28.2 g L^<-1> of xylitol was obtained after 40 h of incubation. The fermentation performance corresponds to 1.3 g L^<-1> h^<-1> volumetric productivity and 0.70 g g^<-1> yield.
一般社団法人日本エネルギー学会の論文
2014-01-08