O-21 ササ稈水解物のキシリトール発酵(セッション5:資源,研究発表(口頭発表))

概要

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To prepare a substrate for microbial conversion of xylose into xylitol, the cuim of Sasa senanensis was hydrolyzed with dilute sulfuric acid. When the reaction temperature was fixed at 121℃, an optimum yield of xylose was obtained by treatment with 2% sulfuric acid for 1 h. Either an increase in the sulfuric acid concentration or a prolonged reaction time resulted in a decrease in the xylose yield. A fermentable substrate with a relatively high xylose concentration (36.7 g IL) was obtained by hydrolysis with 2% sulfuric acid with a liquid to solid ratio of 5 g g^<-1>. During hydrolysis at elevated temperatures, certain undesired byproducts were also generated, such as degradation products of solubilized sugars and lignin, which are potential inhibitors of microbial metabolism. These compounds were, however, successfully removed from the hydrolyzate by treatment with a commercial steam-activated char. Microbial conversion of xylose in the detoxified hydrolyzate into xylitol was carried out using a yeast Candida magnoliae.
2011-01-12