免疫組織化学 : 内分泌病理の立場から(病理領域における新しい解析方法の導入)

概要

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Immunohistochemical methods have played an important role in the pathological research and surgical pathology. This article first overviews the recent progresses of the immunoperoxidase methods, briefly describing on monoclonal antibodies and antibodies against synthetic peptides, three immunostaining methods recently proposed by DAKO (i.e., enhanced polymer one step staining method (EPOS), Envision system as a highly-sensitive indirect method, and catalytic signal amplification (CSA) method), targets of immunostaining, semi-quantitative evaluation of amount of antigen or intensity of staining, and several pitfalls in the immunohistochemistry. Two examples of immunohistochemical studies are then introduced in the endocrine pathology. One is aldosterone synthase and 11β hydroxylase cytochrome P-450 of the adrenal cortex, a hot issue on zona glomerulosa and uppermost zona fasciculata and their transitional zone. The other is immunohistochemical studies of thyroglobulin (TG), focusing on TG derived from papillary carcinoma of the thyroid, TG of autonomously functioning thyroid nodules (AFTN) and nonfunctioning tumors with the surrounding thyroid follicles, and TG as a carrier protein of haptens and synthetic peptides. Finally, intrinsic biotin, an interferer of immunostaining systems containing avidin and biotin, is described as an example of pitfalls of immunohistochemistry and in situ hybridization.
東京女子医科大学の論文
1996-05-25