ラット腎虚血再潅流モデルにおけるCD2AP発現の検討

概要

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CD2-associated protein (CD2AP) is a ubiquitously expressed 80-kDa intracellular protein, and is speculated to act as an intracellular signaling pathway between plasma membrane proteins and cytoskeleton proteins. CD2AP contains three SH3 domains and a coil-coiled domain, motifs that are important in protein-protein interactions. We have utilized an established rat model of ischemic renal injury to investigate CD2AP expression by quantitative real-time PCR for 10 days following ischemia. CD2AP expression increased by up to 4-fold after reperfusion for 6-24 h. Immunoblotting and immunostaining with a polyclonal CD2AP antibody demonstrated a significant increase in CD2AP expression. These results suggest that CD2AP expression increased during the cell proliferation following renal ischemia and that CD2AP may be involved in the process of cell repair and/or differentiation following injury. We also investigated the expression of genes categorized to apoptosis and cell proliferation in M1 cells transfected with full-length CD2AP cDNA. Quantitative real-time PCR showed decreased expression of B-Raf, caspase 12, and CDC2. In conclusion, the results of this study demonstrated that CD2AP expression is significantly up-regulated in adult rat kidney following ischemia-reperfusion injury and that CD2AP might play an important role in the process of cell repair and/or differentiation by suppressing apoptosis and cell proliferation.
東京女子医科大学の論文