Effect of Ethanol on S-Warfarin and Diclofenac Metabolism by Recombinant Human CYP2C9.1(Biopharmacy)
スポンサーリンク
概要
- 論文の詳細を見る
The effect of ethanol on the metabolism of S-warfarin and diclofenac by recombinant cytochrome P450 2C9.1 microsomes (CYP2C9.1) was studied. The 7-hydroxylation metabolism of S-warfarin was inhibited by as low as 0.1vol% (17mM) ethanol. Ethanol decreased the V_<max>/K_m and V_<max> values of S-warfarin metabolism in a concentration-dependent manner, but the K_m value was unchanged by ethanol. The inhibitory effect of ethanol on the 4'-hydroxylation metabolism of diclofenac was not observed even at 1.0vol% (170mM) ethanol. Ethanol at a concentration of 3.0vol% (510mM) increased the K_m value of diclofenac metabolism without changes in the V_<max>, which indicates that diclofenac 4'-hydroxylation by CYP2C9.1 was competitively inhibited by ethanol. S-Warfarin metabolism by CYP2C9.1 was more sensitive to ethanol than diclofenac metabolism. These results suggest that ethanol inhibits the metabolism by CYP2C9.1 in a substrate-dependent manner.
- 公益社団法人日本薬学会の論文
- 2009-03-01
著者
-
IWAKAWA Seigo
Department of Pharmaceutics, Kobe Pharmaceutical University
-
Iwakawa Seigo
伊丹市立伊丹病院 薬剤科
-
Iwakawa Seigo
Department Of Hospital Pharmacy School Of Medicine Kobe University : Department Of Pharmacy Kyoto Un
-
TATSUMI Akitoshi
Educational Center for Clinical Pharmacy, Kobe Pharmaceutical University
-
IKEGAMI Yuki
Department of Pharmaceutics, Kobe Pharmaceutical University
-
MORII Ryoko
Department of Pharmaceutics, Kobe Pharmaceutical University
-
SUGIYAMA Masatoshi
Educational Center for Clinical Pharmacy, Kobe Pharmaceutical University
-
KADOBAYASHI Muneo
Department of Pharmacy, The Hospital of Hyogo College of Medicine
-
Tatsumi Akitoshi
Educational Center For Clinical Pharmacy Kobe Pharmaceutical University:department Of Pharmacy The H
-
Morii Ryoko
Department Of Pharmaceutics Kobe Pharmaceutical University
-
Sugiyama Masatoshi
Educational Center For Clinical Pharmacy Kobe Pharmaceutical University
-
Ikegami Yuki
Department Of Pharmaceutics Kobe Pharmaceutical University
-
Kadobayashi Muneo
Department Of Pharmacy The Hospital Of Hyogo College Of Medicine
-
Iwakawa Seigo
Department of Cardiology, Miki
関連論文
- Antiproliferative Activity of Rhinacanthus nasutus (L.) KURZ Extracts and the Active Moiety, Rhinacanthin C(Pharmacology)
- Effect of 2-Methoxyestradiol, Buthionine Sulfoximine and Hydrogen Peroxide on the Viability of Renal Carcinoma Cell Lines(ACHN and ACVB)(Biopharmacy)
- Effects of Pharmacists' Consultation on Serum Cholesterol Level Using Drug History Notebook or Drug Instruction Sheets for Outpatients with Hypercholesterolemia
- PJ-884 Functional Effects of Renal Stent Placement to Atherosclerotic Renal Artery Stenosis : Factors of Favorable Outcomes(Hypertension, clinical(10)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
- OJ-133 Impact of Reactive Oxygen Species on Plaque Formation and Vessel Remodeling : Comparison of Histochemical Characteristics and IVUS Finding(Atherosclerosis, Clinical 2 (IHD) : OJ16)(Oral Presentation (Japanese))
- OJ-044 Genetic Variant of Cytochrome P450 2C9 in Patients Receiving Warfarin(Antithrombofic Ttherapy (H) : OJ6)(Oral Presentation (Japanese))
- Pharmacokinetics of Pirarubicin in Pediatric Patients
- N-Acetyltransferase2 Genotype Correlated with Isoniazid Acetylation in Japanese Tuberculous Patients
- Effects of Secretable SOD Delivered by Genetically Modified Cells on Xanthine/Xanthine Oxidase and Paraquat-Induced Cytotoxicity in Vitro
- Effects of Cu, Zn-Superoxide Dismutase Delivered by Genetically Modified Skin Fibroblasts on Cold-Induced Skin Edema in Rats
- Protective Effect of Transfection with Secretable Superoxide Dismutase (SOD) (a Signal Sequence-SOD Fusion Protein Coding cDNA) Expression Vector on Superoxide Anion-Induced Cytotoxicity in Vitro)
- Effect of Transfection with a Superoxide Dismutase Expression Plasmid on Xanthine/Xanthine Oxidase-Induced Cytotoxicity in Cultured Rat Lung Cells
- Pharmaceutical Studies for Gene Therapy : Expression of Human Cu, Zn-Superoxide Dismutase Gene Transfected by Lipofection in Rat Skin Fibroblasts
- Effect of Clarithromycin on the Bioavailability of Cyclosporin in Rats
- Effect of Transfection with Superoxide Dismutase Expression Plasmid on Superoxide Anion Induced Cytotoxicity in Cultured Rat Lung Cells
- Pharmacokinetics of Zonisamide ; Saturable Distribution into Human and Rat Erythrocytes and into Rat Brain
- Improvement in Wound Healing by Epidermal Growth Factor (EGF) Ointment. II. Effect of Protease Inhibitor, Nafamostat, on Stabilization and Efficacy of EGF in Burn
- Analysis of Current State Regarding the Use of Antihypertensive Drugs to Outpatients at Internal Medicine Departments of General Hospital
- The Effects of Verapamil on the Gastrointestinal Metabolism of Aprindine
- Chitosan Induces Apoptosis via Caspase-3 Activation in Bladder Tumor Cells
- Effect of Ethanol on the Binding of Warfarin Enantiomers to Human Serum Albumin(Biopharmacy)
- DISPOSITION OF SECRETIN IN RAT STOMACH AND PANCREAS
- Effect of Gender on Theophylline Clearance in the Asthmatic Acute Phase in Japanese Pediatric Patients(Biopharmacy)
- Erratum: Effect of Gender on Theophylline Clearance in the Asthmatic Acute Phase in Japanese Pediatric Patients[Biol. Pharm. Bull. 32(2): 304—307]
- Stereoselective Reductive Metabolism of Metyrapone and Inhibitory Activity of Metyrapone Metabolites, Metyrapol Enantiomers, on Steroid 11β-Hydroxylase in the Rat
- Effect of Oxyethylene Numbers on the Pharmacokinetics of Menatetrenone Incorporated in Oil-in-Water Lipid Emulsions Prepared with Polyoxyethylene-Polyoxypropylene Block Copolymers and Soybean Oil in Rats(Highlighted paper selected by Editor-in-chief,Bioph
- Effect of Ethanol on S-Warfarin and Diclofenac Metabolism by Recombinant Human CYP2C9.1(Biopharmacy)
- Effects of Pharmacists' Consultation on Serum Uric Acid Level in Outpatients with Hyperuricemia
- Effects of Pharmacists' Consultation on Serum Uric Acid Level in Outpatients with Hyperuricemia
- Effect of Glimepiride and Glibenclamide on S-Warfarin 7-Hydroxylation by Human Liver Microsomes, Recombinant Human CYP2C9.1 and CYP2C9.3(Biopharmacy)
- Direct Modulation of Secretin Binding Sites by Gastrin in the Rat Stomach
- Regulation of Epidermal Growth Factor Receptors in Rat Gastric Mucosa : Effect of Gastric Ulceration
- Regulation of Epidermal Growth Factor Receptors in Rat Gastric Mucosa : Effect of Fasting and Sialoadenectomy
- CHANGES IN EGF RECEPTORS ON PLASMA MEMBRANES FROM RAT GASTRIC MUCOSA AND INTERACTION WITH DRUGS
- DISTRIBUTION AND METABOLISM OF SECRETIN AND APROTININ IN ISOLATED PERFUSED RAT PANCREAS
- Different Involvement of DNA Methylation and Histone Deacetylation in the Expression of Solute-Carrier Transporters in 4 Colon Cancer Cell Lines
- Different Effects of Epigenetic Modifiers on the Cytotoxicity Induced by 5-Fluorouracil, Irinotecan or Oxaliplatin in Colon Cancer Cells