Effect of 2-Methoxyestradiol, Buthionine Sulfoximine and Hydrogen Peroxide on the Viability of Renal Carcinoma Cell Lines(ACHN and ACVB)(Biopharmacy)

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2-Methoxyestradiol (2-ME), an endogenous metabolite of 17β-estradiol, induces the intracellular accumulation of superoxide anion (O^・_2^-) and buthionine sulfoximine (BSO) is an inhibitor of glutathione (GSH) synthesis. We have examined the combination anticancer effect of 2-ME and BSO accompanied with hydrogen peroxide (H_2O_2). 2-ME inhibited cell growth in renal carcinoma cell lines (ACHN and ACVB) accompanied by an increase in the intracellular contents of GSH. The combination of 2-ME, BSO and H_2O_2 showed a significant antiprolifer-ation effect in both ACHN and ACVB. The intracellular levels of reactive oxygen species (ROS) with a combination with 2-ME and H_2O_2 in ACHN and ACVB pretreated with BSO were markedly increased, which may have contributed to the potential antiproliferative action.
社団法人日本薬学会の論文
2006-05-01