P-25 メシマコブの低分子微量成分の研究(ポスター発表の部)

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In Japan, many kinds of dietary supplements containing Phellinus linteus ('meshimakobu' in Japanese) are in the markets. These products were prepared from either natural fruit bodies, cultivated fruit bodies, cultured mycelia or the mixture of them, but all of them are used for same purposes. We supposed that there must be differences in the chemical constituents among them and these differences may lead to variation in the biological activities. We compared the HPLC profiles of the MeOH extracts prepared from authentic P. linteus natural fruit body, cultured mycelia and cultivated fruit bodies. Their HPLC chromatograms were different from each other. The HPLC profiles of the extracts from the fruit bodies cultivated on the two different host species were remarkably similar. We isolated compounds corresponding to peaks characteristic to the natural fruit body extract and determined their structures as the novel fused aromatic compounds, meshimakobnol A (1) and B (2) by spectroscopic methods including the detection of longer-range C-H coupling constants (^<4,5>J_<C-H>) An additional new compound, phellifuropyranone A (3) was also isolated together with known compounds, phelligridin E (4), G (5) and I (6), from natural fruit body. The characteristic compounds in cultivated fruit bodies were isolated and identified as known compounds, hypholomine B (7) and inoscavin A (8). Next, we measured the content of meshimakobnol A (1) in authentic P. linteus natural fruit body, commercially available natural and cultivated fruit bodies and cultured myceria. The content of 1 in the authentic P. linteus natural fruit body was 0.08% and 0.06 to 0.14% in the commercially available products from natural fruit body origin. In contrast, the cultivated fruit body also contained 1 but only in very small amount, 0.021%. 1 could not be detected in cultured mycelia. These results meshimakobnol A (1) could become one of indicators for discriminating between the natural and those from cultivated fruit bodies or cultured mycelia. Meshimakobnol A (1) showed proliferation inhibiting activity against the mouse melanoma cells and human lung cancer cells in vitro.
天然有機化合物討論会の論文
2007-08-24

P-25 メシマコブの低分子微量成分の研究(ポスター発表の部)

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