P-65 アーバスキュラー菌根菌の宿主認識シグナル物質Branching Factorの同定(ポスター発表の部)

概要

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Arbuscular mycorrhizal (AM) fungi form mutualistic symbiotic associations with the roots of more than 80% of land plants. The fungi are incapable of completing their life cycle in the absence of a host root. Their spores can germinate and grow in the absence of a host, but their hyphal growth is very limited. Little is known about the molecular mechanisms governing signalling and recognition between AM fungi and host plants. Hyphae of AM fungi show extensive branching in the vicinity of host roots prior to appressorium formation. This hyphal morphogenesis has long been described as one of the first events in host recognition by the fungus during pre-infection stages. Host roots release the signaling molecules for hyphal branching, but the active compounds, called "branching factors (BFs)" have not been isolated. Here we have isolated a BF from root exudates of Lotus japonicus, and identified this compound as a strigolactone, 5-deoxy-strigol, by spectroscopic analysis and chemical synthesis. Strigolactones are a group of sesquiterpene lactones, previously isolated as seed germination stimulants for the parasitic weeds Striga and Orobanche. Natural strigolactones, 5-deoxy-strigol, sorgolactone, and strigol as well as a synthetic analogue GR24 induced extensive hyphal branching in germinating spores of the AM fungus Gigaspora margarita at nanogram to picogram levels in the paper disc diffusion method.
天然有機化合物討論会の論文
2005-09-15