107(P-50) 興奮性アミノ酸ダイシハーベインの組織内局在性 : 産生生物と存在意義の解明に向けて(ポスター発表の部)

概要

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Cellular localization of the marine sponge-derived excitatory amino acid dysiherbaine (1) was investigated using immunohistochemical methods. Dysiherbaine (1), isolated from a sponge Dysidea herbacea, selectively activates mammalian non-NMDA type glutamate receptors (GluRs) and induces seizure in mice. Recently 1 was found to have unusually high affinity towards GluR5 and 6, subtypes of kainite-type GluRs, and can activate these subtype receptors selectively in the heteromerically expressed receptor complex. These unusual actions of 1 to the neuronal receptors warrants its usefulness as a research tool, however, its production, biosynthesis, and roles in the natural environment are left to be investigated. Since microorganisms including a cyanobacterial, Oscillatoria spongeliae are known to be heavily associated with D. herbacea, actual producers of the secondary metabolites and their roles in the sponge and associated microorganisms are difficult problem to solve. In this study, we paved a way to approach this elusive problems by showing cellular localization of 1 using immnumohistochemical methods. We also show a presence of putative DH-binding protein of 111kD using DH-conjugated affinity chromatography and SDS-PAGE.
天然有機化合物討論会の論文
2003-09-01