P-52 ココナッツミルク中の主サイトカイニンの分離と構造決定(ポスター発表の部)

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Cytokinins are plant hormones which regulate plant cell proliferation. One of the classical sources of cytokinin activity is coconut milk, the fluid endosperm of coconut (Cocos nucifera L.). A number of attempts to identify cytokinins in coconut milk have been reported. 1,3-Diphenylurea was isolated as a factor, though it has not yet been established whether the compound is really produced by the plant or is an artifact generated during the extraction procedure. It has also been reported that coconut milk contains potent cytokinins such as zeatin, and zeatin riboside (ZR). However, the contents of these known cytokinins, as well as the reported content of 1,3-diphenylurea, are quite low, and less than 1% of the cytokinin activity of coconut milk can be accounted for by these known cytokinins. We reported here the isolation and structural identification of a major cytokinin in coconut milk. The progress of the isolation was monitored by use of the tobacco callus growth-promoting assay. The structure of the factor was determined to be 14-O-{3-O-[β-D-galactopyranosyl-(1→2)-α-D-galactopyranosyl-(1→3)-α-L-arabinofuranosyl]-4-O-(α-L-arabinofuranosyl)-β-D-galactopyranosyl}-trans-zeatin riboside [G_3A_2-ZR] by various NMR techniques, including HMBC, as well as mass spectroscopy and sugar analysis. The optimum concentration of G_3A_2-ZR for cytokinin activity in the tobacco callus assay was estimated to be 5×10^<-6> M, so that G_3A_2-ZR is one order more potent than 1,3-diphenylurea and one order less potent than zeatine riboside. At least 20% of the cytokinin activity of coconut milk could be attributed to G_3A_2-ZR.
天然有機化合物討論会の論文
1995-09-01

P-52 ココナッツミルク中の主サイトカイニンの分離と構造決定(ポスター発表の部)

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