酵母Three-hybrid systemを用いたサイトカイニン結合タンパク質の探索

概要

論文の詳細を見る
Cytokinins regulate several physiological phenomena in plant growth. To study the mechanism of the action of this hormone in different phenomena, identification of cytokinin binding proteins and receptor genes have been extensively studied, and several binding proteins and a putative receptor gene are reported. We tried to screen genes encoding cytokinin binding protein using the yeast three-hybrid system, which can detect ligand-receptor interaction. As the bait, we synthesized a heterodimer of bait and to test the system, we prepared a control strain harboring a plasmid which expresses CSBP-B42 fusion protein. We could not detect the expression of covalently-linked dexamethasone and N-phenyl N'-(4-pyridyl) urea. To confirm the balidity of the reporter gene in this positive control system. Since the bait didn't show any activity in Amaranthus bioassay, the interaction between the bait and CSBP wouldn't be high enough. Improvement of the system including the rebuilding of a new bait is now under way.
植物化学調節学会の論文
2000-11-02