4 抗生物質生産誘導因子Virginiae Butanolide Aの生合成(口頭発表の部)

概要

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Virginiae butanolide A (VB-A) is one of the virginiamycin inducing factors isolated from the culture broth of Streptomyces virginiae, and has a unique 2,3-disubstituted butanolide skeleton which is common in other signal molecules in Streptomyces. The biosynthesis of VB-A in S. antibioticus, which was found as a high producer of VB-A, was studied by feeding experiments with labeled precursors. ^<13>C NMR and CI-MS analysis of the VB-A dibenzoate samples obtained by feeding experiments with sodium [1-^<13>C]- and [2-^<13>C]acetate, sodium [1-^<13>C]isovalerate and [1,3-^<13>C]glycerol showed that the VB-A molecule originated from two moles of acetic acid and each mole of isovaleric acid and glycerol. Further feeding experiments with ^<13>C-double labeled N-acetylcysteamine thioester of β-ketoacid (1) and glycerol-d_5 indicated that a probable biosynthetic pathway of VB-A is a coupling between a β-ketoacid derivative and a C_3 unit from glycerol, such as dihydroxyacetone or its derivatives.
天然有機化合物討論会の論文
1990-09-25