放線菌信号伝達物質の構造と機能

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Actinomycetes are Gram-positive bacteria charactesized by their versatile ablility to produce secondary metabolites. One of the most interesting features of these microorganisms is their ability to produce endogenous and exogeneous signal molecules which control both the cytodifferentiation and secondary metabolism. These have sometimes been called "autoregulators", and so far 10 autoregulators have been isolated from Streptomyces species and their structures elucidated. Among them, we have found 6 signal molecules, i.e., virginiae butanolide A, B, C, D, E which trigger viginiamycin production of Streptomyces virginiae and IM-2 which triggers blue pigment production by Streptomyces sp. FRI-5. To know the complex regulation mechanism on secondary metabolism in Streptomyces species, signal transmission mechanism involved in the autoregulators seems important. This paper summarizes our previous work, and the following findings are discussed. i) Isolation and structure elucidation of virginiae butanolides from Streptomyces virginiae. Virginiae butanolides are effective even at 1 ng/ml in initiating virginiamycin production. ii) Isolation and structure elucidation of IM-2 from Streptomyces sp. FRI-5. IM-2 is effective at 0.6 ng/ml in initiationg blue pigment production, and shares the same butyrolactone moiety as virginiae butanolides, but is more hydrophilic due to its shorter substituent at position 2. iii) The distribution of virginiae butanolides and IM-2 among Streptomyces species. Virginiae butanolides and IM-2 are found to be distributed in about 14% and 11%, respectively, of antibiotic producting Streptomyces species. iv) The relationship between structure and activity of virginiae butanolide C using 41 synthetic analogs to clarify the essential moiety in its structure. v) Identification, purification and characterization of virginiae butanolide-binding protein which seems to mediate pleiotropic signal transmitted by virginiae butanolides in Streptomyces virginiae.
社団法人日本生物工学会の論文
1991-03-25

放線菌信号伝達物質の構造と機能

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