Trehalose Is an Important Mediator of Cap1p Oxidative Stress Response in Candida albicans(Microbiology)

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Trehalose, a nonreducing disaccharide which accumulates dramatically during stationary phase or under oxidative stress, is well known as a stress protectant in several organisms. Here we investigated the putative correlation of trehalose with Caplp, which is a basic region-leucine zipper (bZip) transcription factor participating in oxidative stress tolerance in Candida albicans. HPLC-MS analysis showed that trehalose did not accumulate in the capllcapl mutant during stationary phase. When the mutant was exposed to high concentration of H_2O_2, trehalose accumulation was still not induced. Under both of the conditions above, the capllcapl mutant showed high sensitivity to H_2O_2, and the cell viability was rather low. Furthermore, when exogenous trehalose was added to the culture of the capllcapl mutant, the tolerance of this strain to oxidative stress was increased. Real time reverse transcription polymerase chain reaction (RT-PCR) analysis revealed that the transcript levels of TPS2 and TPS3 were increased in the wild type strain compared to that in capllcapl mutant when exposed to H_2O_2. These results indicated that trehalose accumulation is important to the oxidative stress tolerance mediated by Caplp in C. albicans.
公益社団法人日本薬学会の論文
2008-03-01