Inhibition of Human Cytomegalovirus Infection by IE86-Specific Short Hairpin RNA-Mediated RNA Interference
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概要
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To develop a gene therapeutic method for human cytomegalovirus (HCMV), the IE86 specific short hairpin (sh) RNA expressing vector was constructed and subsequently transfected into MRC-5 cells. After infection of these cells with HCMV AD169, expression of IE86 was reduced strikingly as compared to the control. In addition, the inhibitory effect corresponded to a decrease in viral DNA replication and the virus-induced cytopathic effect. Measurement of the virus yield demonstrated that infection of cells expressing IE86-specific shRNA resulted in suppression of the formation of infectious viral progeny. These observations indicate that IE86 can be used as an effective target against HCMV infection using RNA interference (RNAi) technology, which provides new possibilities for anti-HCMV studies.
著者
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WANG Bin
Department of Electrical and Electronic Engineering, Akita University
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Li Ling
Department of Cardiology, the First Affiliated Hospital, Zhengzhou University
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LIU ZHIJUN
Department of Chemical Equipment and Control Engineering, School of Chemical Engineering, Dalian Uni
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BAI Zhiqiang
Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao Univers
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LIU Haiyan
Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao Univers
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JIANG Guangyu
Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao Univers
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WANG Haitao
Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao Univers
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YAN Zhiyong
Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao Univers
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QIAN Dongmeng
Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao Univers
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DING Shouyi
Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao Univers
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SONG Xuxia
Department of Microbiology, Key Laboratory of Medicine and Biotechnology of Qingdao, Qingdao Univers
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