Inhibition of Human Cytomegalovirus Infection by IE86-Specific Short Hairpin RNA-Mediated RNA Interference
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概要
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To develop a gene therapeutic method for human cytomegalovirus (HCMV), the IE86 specific short hairpin (sh) RNA expressing vector was constructed and subsequently transfected into MRC-5 cells. After infection of these cells with HCMV AD169, expression of IE86 was reduced strikingly as compared to the control. In addition, the inhibitory effect corresponded to a decrease in viral DNA replication and the virus-induced cytopathic effect. Measurement of the virus yield demonstrated that infection of cells expressing IE86-specific shRNA resulted in suppression of the formation of infectious viral progeny. These observations indicate that IE86 can be used as an effective target against HCMV infection using RNA interference (RNAi) technology, which provides new possibilities for anti-HCMV studies.
- 2010-07-23
著者
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Li Ling
Department of Cardiology, the First Affiliated Hospital, Zhengzhou University
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Liu Zhijun
Department Of Chemical Equipment And Control Engineering School Of Chemical Engineering Dalian Unive
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Wang Bin
Department Of Electrical And Electronic Engineering Akita University
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Li Ling
Department Of Microbiology Key Laboratory Of Medicine And Biotechnology Of Qingdao Qingdao Universit
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Liu Haiyan
Department Of Microbiology Key Laboratory Of Medicine And Biotechnology Of Qingdao Qingdao Universit
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Wang Haitao
Department Of Microbiology Key Laboratory Of Medicine And Biotechnology Of Qingdao Qingdao Universit
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Wang Bin
Department Of Microbiology Key Laboratory Of Medicine And Biotechnology Of Qingdao Qingdao Universit
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Liu Zhijun
Department Of Microbiology Key Laboratory Of Medicine And Biotechnology Of Qingdao Qingdao Universit
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Song Xuxia
Department Of Microbiology Key Laboratory Of Medicine And Biotechnology Of Qingdao Qingdao Universit
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Yan Zhiyong
Department Of Microbiology Key Laboratory Of Medicine And Biotechnology Of Qingdao Qingdao Universit
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Ding Shouyi
Department Of Microbiology Key Laboratory Of Medicine And Biotechnology Of Qingdao Qingdao Universit
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Bai Zhiqiang
Department Of Microbiology Key Laboratory Of Medicine And Biotechnology Of Qingdao Qingdao Universit
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Qian Dongmeng
Department Of Microbiology Key Laboratory Of Medicine And Biotechnology Of Qingdao Qingdao Universit
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Jiang Guangyu
Department Of Microbiology Key Laboratory Of Medicine And Biotechnology Of Qingdao Qingdao Universit
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Wang Bin
Department Of Chemistry Marshall University
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Wang Haitao
Department Of Metallurgical Engineering University Of Utah
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