タンパク質担持水酸アパタイト顆粒の乳酸・グリコール酸共重合体によるカプセル化(秋季シンポジウム若手研究者論文)

概要

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A solid-in-oil-in-water (S/O/W) emulsion-solvent evaporation method was applied to encapsulate protein-loaded hydroxyapatite (HAp) granules (D_<50>=19.5μm) with a biodegradable polymer of poly (lactic-co-glycolic acid) (PLGA). After loading bovine selum albumin (BSA), the HAp granules were mechanically dispersed in oil phase (PLGA dissolved in CH_2Cl_2), and then the suspension was dropped into water containing an emulsion stabilizer of polyvinyl alcohol (PVA). Stirring of the dropped suspension in the water phase produced S/O/W emulsions. The emulsions were diluted with excess water to facilitate removal of the organic solvent (CH_2Cl_2) from the oil phase. After the evaporation of CH_2Cl_2, the particulate materials were separated, washed and vacuum-dried. The resultant particles had very smooth PLGA surface with spherical shape, and their mean particle size was about several microns larger than that of the HAp granules. Fluorescently-labeled confocal laser scanning microscopy (CLSM) revealed that the protein-loaded HAp granules were encapsulated in the PLGA microsphere.
社団法人日本セラミックス協会の論文
2007-11-01