SIMULTANEOUS MEASUREMENT OF NUCLEATED CELL COUNTS AND CELLULAR DIFFERENTIALS IN RAT BONE MARROW EXAMINATION USING FLOW CYTOMETER
スポンサーリンク
概要
- 論文の詳細を見る
The purpose of this study was to establish the simultaneous measurement of nucleated cell counts and cellular differentials in rat bone marrow examination. The bone marrow cells were stained with an anthraquinone fluorescent DNA stain (DRAQ5) and fluorescence-labeled antibodies, and were analyzed quantitatively using a flow cytometer in the presence of internal standard beads. DRAQ5 distinguished populations of nucleated cells. The absolute counts of nucleated cells were determined using an internal standard, and were equivalent to that measured by the electrical resistance method. The population of nucleated cells was classified into myeloids and erythroids by labeling with CD11b/c and CD71 antibodies, respectively. In a separate examination, T- and B-lymphocytes were also classified by labeling with CD3 and CD45RA antibodies, respectively. The classification of each cell lineage was identical with that of the alternative flow-cytometric method in which cells were differentiated according to cellular size and the fluorescence of a peroxidase indicator, 2',7'-dichlorofluorescin. The ratios of cell lineage, together with myeloid/erythroid ratio (ME), were the same as those obtained by a manual microscopic method. The present flow cytometric method enables the simultaneous measurement of the total nucleated cell counts and cellular differentials of rat bone marrow cells, allowing for rapid and highly quantitative bone marrow examination in rats.
- 日本トキシコロジー学会の論文
- 2007-08-17
著者
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IIDAKA TAKESHI
Drug Safety Evaluation, Nagoya Laboratories, Pfizer Global Research and Development
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HORII IKUO
Drug Safety Evaluation, Nagoya Laboratories, Pfizer Global Research and Development
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Iidaka Takeshi
ファイザー 中央研究所
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Iidaka Takeshi
Drug Safety Research And Development Pfizer Global Research And Development Nagoya Laboratories. Pfi
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Iidaka Takeshi
ファイザー製薬中央研究所 安全性研究統括部
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Iidaka Takeshi
Drug Safety Research And Development Nagoya Laboratories Pfizer Global Research And Development Pfiz
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Iidaka Takeshi
ファイザー製薬中央研究所 安全性研究
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Horii Ikuo
Showa Univ. (department Of Biochemical Toxicology School Of Pharmaceutical Sciences)
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Horii I
Drug Safety Research And Development Pfizer Global Research And Development Nagoya Laboratories. Pfi
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Horii Ikuo
Pfizer Global Research & Development Nagoya Laboratories Pfizer Inc.
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HAMADA Yoshimasa
Drug Safety Research and Development, Nagoya Laboratories, Pfizer Japan Inc.
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Kurata Masaaki
ファイザー製薬中央研究所 安全性研究統括部
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Kurata Masaaki
Drug Safety Research And Development Nagoya Laboratories Pfizer Global Research And Development Pfiz
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Horii Ikuo
Worldwide Safety Sciences Pgrd Nagoya Laboratories Pfizer Inc.
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Horii Ikuo
Worldwide Safety Sciences Pfizer Global Research & Development Nagoya Laboratories Pfizer Inc.
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Hamada Y.
Drug Safety Research And Development Pfizer Global Research And Development Nagoya Laboratories. Pfi
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Horii Ikuo
Drug Safety Evaluation Pfizer Global Research And Development
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Horii Ikuo
Drug Safety Evaluation Global Research & Development Pfizer Inc.
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Horii Ikuo
Biochemical Toxicology School Of Pharmaceutical Sciences Showa University
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Hamada Yoshimasa
Drug Safety Research And Development Nagoya Laboratories Pfizer Japan Inc.
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Kurata Masaaki
Drug Safety Research And Development Nagoya Laboratories Pfizer Global Research And Development Pfiz
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