FLOW CYTOMETRIC ANALYSIS FOR SPERM VIABILITY AND COUNTS IN RATS TREATED WITH TRIMETHYLPHOSPHATE OR PYRIDOXINE
スポンサーリンク
概要
- 論文の詳細を見る
Six-week old SD-Slc male rats were treated for 4 weeks with compounds known to induce toxicological changes in male reproductive organs (pyridoxine in saline, 500 mg/kg/day, i.p.) or sperm (trimethylphosphate in distilled water, 100 mg/kg/day, p.o.). Each sperm sample taken from the cauda epididymis was analyzed with flow cytometry for the evaluation of sperm viability and counts. Sperm motility and morphology by microscopical observation, and histopathological examination of reproductive organs were also performed for estimating the adverse effects of each compound on spermatogenesis and sperm. While a decrease in sperm motility was noted for the trimethylphosphate group, the low motile sperm was evaluated as being viable sperm, not moribund, with flow cytometry. In the pyridoxine group, microscopical observation revealed morphological changes of sperm and a decrease of motility. The present sperm analysis with flow cytometry also suggested morphological changes reflected by dot plot as well as decrease of sperm viability and counts. These results indicated that this procedure led to profound findings in the compound-treated animals, with evaluation as viable sperm, not moribund, in a low motile sperm sample, and suggesting morphological changes in the dot plot of flow cytometry.
- 日本トキシコロジー学会の論文
- 1998-02-13
著者
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KATOH CHIAKI
Department of Internal Medicine, National Hospital Organization Nagoya Medical Center
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Horii Ikuo
Department of Toxicology and Pathology, Nippon Roche K.K.Research Center
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Horii Ikuo
Dept. Of Preclinical Science Nippon Roche Research Center
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Horii Ikuo
Department Of Preclinical Science Nippon Roche K. K. Research Center
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Katoh C
Nippon Roche K. K. Kanagawa Jpn
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Inomata Akira
Department Of Physics State University Of New York-albany
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Inomata Akira
Department Of Physics State University Of New York At Albany
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Horii Ikuo
Worldwide Safety Sciences Pgrd Nagoya Laboratories Pfizer Inc.
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TAKIZAWA Setsuko
Dept. of Preclinical Science, Nippon Roche Research Center
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TAKIZAWA Setsuko
Department of Toxicology and Pathology, Nippon Roche Research Center
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Inomata A
Drug Safety Res. Laboratories (tsukuba) Eisai Co. Ltd.
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Takizawa Setsuko
Dept. Of Preclinical Science Nippon Roche Research Center
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Takizawa Setsuko
Department Of Preclinical Science Nippon Roche K. K. Research Center
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Takizawa Setsuko
日本ロシュ
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Horii Ikuo
Department Of Biochemical Toxicology School Of Pharmacy Showa University
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