Single Site Proteolysis in Silkworm Antitrypsin Causes Structural Changes in Behavior against Denaturing Reagents(Biological Chemistry)

スポンサーリンク

概要

• 論文の詳細を見る

• Silkworm antitrypsin (sw-AT), which was thought to belong to serpin family, changed its behavior against denaturation after chymotryptic cleavage of a single peptide bond (Tyr-Val) two amino acids away from the reactive site for trypsin (Lys-Val). This chymotrypsin-modified sw-AT became resistant to denaturation by heat, sodium dodecyl sulfate, or guanidine hydrochloride, and this characteristic was evident in its circular dichroism spectrum. The modified sw-AT was also indigestible by S. aureus V8 protease. These facts should indicate a structural change from a stressed, unstable state to a stable one accompanying the cleavage of the single peptide bond in sw-AT. The stabilizing factor was in part attributed to the interaction of a COOH-terminal fragment (5kDa) and an NH_2-terminal one (36kDa) in modified sw-AT.

• 社団法人日本農芸化学会の論文
• 1990-01-23

著者

• Kobayashi Kazuto

  Department of Research and DevelopmentHonda, Electronics Co., Ltd.

• Sasaki Takuji

  Department Of Molecular Genetics National Institute Of Agrobiological Sciences

• Sasaki T

  Nagoya Univ. Aichi Jpn

• KOHARA Atuko

  Department of Food Science and Technology, School of Agriculture, Nagoya University

• SHIMIDZU Takaaki

  Department of Food Science and Technology, School of Agriculture, Nagoya University

• Kobayashi Kazuto

  Department Of Cardiovascular Medicine Okayama University

• Kobayashi Kazuto

  Department Of Food Science And Technology School Of Agriculture Nagoya University

• Kohara Atuko

  Department Of Food Science And Technology School Of Agriculture Nagoya University

• Shimidzu Takaaki

  Department Of Food Science And Technology School Of Agriculture Nagoya University

• Sasaki Takuji

  Department Of Food Science And Technology Faculty Of Agriculture Nagoya University

関連論文

• 9-31 cDNAマイクロアレイによるオオムギの亜鉛欠乏誘導性遺伝子群の解析(9.植物の無機栄養)
• (2)イネcDNAマイクロアレイを用いたいもち病菌接種後の宿主遺伝子の発現解析(平成15年度日本植物病理学会大会講演要旨)
• 10-6 コムギ葉の老化過程におけるRubisco分解 : 免疫電顕とcDNAマイクロアレイによる解析(10.植物の代謝)
• On the 60^ Anniversary of Breeding Science
• イネ日本型品種における食味の粘りおよび外観に関与する量的形質遺伝子座の検出
• 発生・進化メカニズム イネゲノム全塩基配列情報の獲得--イネ科穀類ロゼッタストーン解読の鍵 (ゲノムから生命システムへ) -- (ゲノムから生物学)
• イネの出穂期に関与する量的形質遺伝子座Hd4およびHd5の連鎖解析と特徴づけ
• イネゲノム構造と機能解明の最先端
• イネゲノム塩基配列国際コンソーシアムが37,000個の遺伝子を解析
• イネゲノム塩基配列完全解読の達成と今後のイネ研究に対する意義
• FAO・IRRI主催国際コメ年記念科学論文コンテスト最優秀賞受賞記念論文 21世紀の農業技術戦略におけるイネゲノム塩基配列情報の利用 (21世紀の農業技術開発戦略の研究)
• (59) いもち病菌接種や傷害によって誘導されるイネペルオキシダーゼ遺伝子群の発現特異性(平成17年度日本植物病理学会大会講演要旨)
• S083 マイクロアレイ解析 : オリゴマイクロアレイとその利用について(小集会)
• Paradoxical Brain Embolism with Klippel-Trenaunay Syndrome
• Identification of Several Rice Genes Regulated by Si Nutrition(Plant Nutrition)
• Upregulation of the Genes for Ferritin, RNase, and DnaJ in Leaves of Rice Plants in Response to Sulfur Deficiency(Plant Nutrition)
• OJ-224 Enhancement of the Elastisity of Myofibers in Cardiac Myocytes Associated with Dilated Cardiomyopathy(Cardiomyopathy, basic/clinical-2 (M) OJ38,Oral Presentation (Japanese),The 70th Anniversary Annual Scientific Meeting of the Japanese Circulation
• Prevalence of Atrial Fibrillation in Community-Dwelling Japanese Aged 40 Years or Older in Japan : Analysis of 41,436 Non-Employee Residents in Kurashiki-City
• イネ、その全ゲノム塩基配列解析と遺伝資源の活用
• バイオインフォマティクスの広がり(2)育種からバイオインフォマティクスを見る
• Hd3a, a Rice Ortholog of the Arabidopsis FT Gene, Promotes Transition to Flowering Downstream of Hd1 under Short-Day Conditions
• Large-Scale Identification of Elicitor-Responsive Genes in Suspension-Cultured Rice Cells by DNA Microarray
• SELECTIVE COLLECTION OF CATECHOLAMINERGIC (CA) NEURONS IN THE BRAIN AND ITS APPLICATION TO GENE EXPRESSION ANALYSES
• SELECTIVE COLLECTION OF CATECHOLAMINERGIC (CA) NEURONS IN THE BRAIN AND ITS APPLICATION TO FUNCTIONAL ANALYSES USING TYROSINE HYDROXYLASE (TH)-GREEN FLUORESCENT PROTEIN (GFP) TRANSGENIC MICE
• Purification and Properties of the Endo-1,4-β-Glucanase from Ruminococcus albus and Its Gene Product in Escherichia coli
• Single Site Proteolysis in Silkworm Antitrypsin Causes Structural Changes in Behavior against Denaturing Reagents(Biological Chemistry)
• Limited Proteolysis of Silkworm Antitrypsin by Several Proteinases(Biological Chemistry)
• Purification and Properties of a New endo-Cellulase from Robillarda sp. Y-20(Biological Chemistry)
• FRS-087 Relationship between SCN5A Mutation and Atrial Vulnerability in Brugada Syndrome(Arrhythmia (clinical) ; Pathophysiology of Atrial Fibrillation, The 71st Annual Scientific Meeting of the Japanese Circulation Society)
• THE ROLE OF CENTRAL NORADRENERGIC (NA) SYSTEM IN NEUROENDOCRINE RESPONSES FOLLOWING INTRAPERITONEAL ADMINISTRATION OF LIPOPOLYSACCHARIDE (LPS)
• Mouse liaison for integrative brain research
• Microarray Analysis of Sink-Source Transition in Rice Leaf Sheaths
• Novel Gene Encoding a Ca^-Binding Protein and under Hexokinase-dependent Sugar Regulation(Biochemistry & Molecular Biology)
• cDNA microarray analysis of gene expression in rice plants treated with probenazole, a chemical inducer of disease resistance
• End Sequencing and Chromosomal in silico Mapping of BAC Clones Derived from an indica Rice Cultivar, Kasalath
• Fine Mapping and Characterization of Quantitative Trait Loci Hd4 and Hd5 Controlling Heading Date in Rice
• イネゲノム塩基配列と国際共同研究 (イネゲノム解析研究特集)
• 国際イネゲノム全塩基配列解読プロジェクト(IRGSP)の足跡
• 革新的育種法で次世代農業に活路を (特集 いま、未来の農業を追う)
• 植物ゲノム研究最前線--遺伝子組換えアレルギーからの脱却を
• From Mapping to Sequencing, Post-sequencing and Beyond
• Dopamine deficiency in mice
• Structure, Heterologous Expression, and Properties of Rice (Oryza sativa L.) Family 19 Chitinases(Biochemistry & Molecular Biology)
• Next generation sequencing : Gold mine or tsunami for breeding science
• Polyol-induced Stabilization of a Cold-labile Protein(Biological Chemistry)
• Controlled cell targeting system to study the brain neural circuitry
• Good eating quality : Practically attainable but still genetically unsolved
• Breeding Science in the Genomics Era
• Rice Genome Analysis : Understanding the Genetic Secrets of the Rice Plant
• Making a difference in breeding science
• Bridging the gap between genomics and breeding
• Legacy derived from Norin 10
• Darwinism to Breeding
• Genetic evidence for noradrenergic control of long-term memory consolidation
• DaizuBase, in integrated soybean genome database including BAC-based physical maps
• Steps toward greater internationalization
• Breeding and Biodiversity
• Completion of Rice Genome Sequencing:—a Paradigm Shift of Rice Biology—
• Cleavage Site Specificities of Silkworm Alkaline Proteases
• Estimation of Surface Excess of Surfactant under Coexisting Salts in Foam Separation

もっと見る 閉じる

スポンサーリンク