10504 幹細胞が自己生成したコラーゲンマトリックスの組成・強度変化(Visual Presentation Contest)

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Porcine synovial membranes were obtained from the knee joints, and synovium-derived stem cells were cultured in monolayer with ascorbic acid 2-phosphate to promote the synthesis of extracellular matrix proteins. The synthesized matrix including the stem cells was manually released from the substratum. The detached matrix was then allowed active contraction for 8 hours to develop a 3-dimentional structure. Histological and immunohistochemical analyses were performed to determine the micro structure and chemical composition of the matrix. The matrix was then subjected to a tensile testing at a rate of 0.05mm/s in saline solution at 37℃ using a micro tensile tester developed in our laboratory. Results revealed that the active contraction formed the matrix to a multilayered structure consisting of Type I/III collagen for elastic property, and fibronectin and vitronectin for adhesive property. The tensile test revealed that the tensile strength of the matrix became higher with the increase of tissue culture period in case of the addition of ascorbic acid; the strength and strain of failure of the matrix were 400kPa and 40%, respectively, at 14 days of tissue culture period.
社団法人日本機械学会の論文
2005-03-17