546 3次元scaffold-free組織により再生された軟骨のマイクロ力学特性(S09-3 関節のバイオメカニズム(3),S09 関節のバイオメカニズム)

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Synovium-derived cells from porcine stifle joints were cultured in DMEM. Seven days after the injection of ascorbic 2-phosphate, the cultured tissue were allowed active contraction for 8 hours to form 3-D scaffold-free synthetic tissues (3DSTs). The 3DST was implanted to a round shaped defect of porcine femoral cartilage, a cylindricallyshaped cartilage-subchondral bone specimen was extracted. An atomic force microscopy (AFM) was utilized for the determination of cartilage surface geometry and force-curve indentation test. Results revealed that surface roughness of the cartilage regenerated with 3DST was 298nm, indicating a slight increase as compared with that of intact cartilage. The stiffness of the cartilage regenerated with 3DST was significantly lower than that of intact cartilage, which was approximately 1/4 of that of intact cartilage. It was suggested that these morphological and mechanical changes were related to the synthetic of new collagen fibers and matrix in the regenerated cartilage. It was also suggested that the decrease in stiffness of the regenerated cartilage from intact cartilage was more significant in micro level than in macro level.
社団法人日本機械学会の論文
2006-09-15