Dentin Phosphophoryn Accelerates rhBMP-2/HAP-induced Bone Formation
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概要
- 論文の詳細を見る
Dentin phosphophoryn (DPP) is an acidic non-collagenous protein in dentin. In vitro, DPP induces apatite formation when immobilized to insoluble substrates such as type I collagen fibrils and agarose beads. In the present study, DPP purified from fresh bovine dentin was evaluated as a co-factor for recombinant human bone morphogenetic protein-2 (rhBMP-2) in rhBMP-2-induced bone formation in rats. Five micrograms of Escherichia coli-derived rhBMP-2 variant was adsorbed to composite of 50mg of hydroxyapatite (HAP) with or without 1mg of DPP. Next, rhBMP-2/HAP/DPP composite or rhBMP-2/HAP composite was implanted subcutaneously in 4-week-old Wistar rats. Rats were sacrificed at 2 and 3 weeks after implantation. Throughout the experimental period, rhBMP-2/HAP/DPP composite induced more bone formation than rhBMP-2/HAP composite. In the histomorphological analysis, bone induced by rhBMP-2/HAP/DPP composite accounted for 69±17% (n=6) of the total composite while accounting for 45±13% (n=6) of the rhBMP-2/HAP composite at 3 week postimplantation. These results indicate that the bone-inducing activity of rhBMP-2 is enhanced by DPP as a co-factor of rhBMP-2 in vivo.
- 日本再生歯科医学会の論文
- 2004-03-30
著者
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Saito Takashi
Department of Child Health, Institute of Clinical Medicine, University of Tsukuba
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YOSHIYAMA Masahiro
Department of Operative Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pha
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小池 俊之
北医療大・歯・う蝕
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BESSHO Kazuhisa
Department of Oral and Maxillofacial Surgery, Graduate School of Medicine, Kyoto University
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KOBAYASHI Fumio
Department of Health and Psychosocial Medicine, Aichi Medical University
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Yoshiyama Masahiro
Department Of Operative Dentistry Okayama University Graduate School Of Medicine And Dentistry
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Yoshiyama Masahiro
Department Of Conservative Dentistry Tokushima University School Of Dentistry
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Uemura Toshimasa
Age Dimension Research Center National Institute Of Advanced Industrial Science And Technology
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KOIKE Toshiyuki
Department of Chemistry and Biotechnology, Yokohama National University
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Koike Toshiyuki
Department Of Operative Dentistry And Endodontology School Of Dentistry Health Sciences University O
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Koike Toshiyuki
Department Of Chemistry And Biotechnology Yokohama National University
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Bessho Kazuhisa
Department Of Oral And Maxillofacial Surgery Faculty Of Medicine Kyoto University
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IZUMIKAWA Masanobu
Department of Operative Dentistry and Endodontology, School of Dentistry, Health Sciences University
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Kobayashi Fumio
Department Of Health And Psychosocial Medicine Aichi Medical University School Of Medicine
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Kobayashi Fumio
Department Of Operative Dentistry And Endodontology School Of Dentistry Health Sciences University O
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Kobayashi Fumio
Department Of Health And Psychosocial Medicine Aichi Medical University
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Izumikawa Masanobu
Department Of Operative Dentistry And Endodontology School Of Dentistry Health Sciences University O
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Saito Takashi
Department Of Applied Chemistry Faculty Of Engineering Gunma University
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Saito Takashi
Department Of Operative Dentistry And Endodontology School Of Dentistry Health Sciences University O
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Saito Takashi
Department Of Agronomy Faculty Of Agriculture Tohoku University
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