Influence of Allosteric Effectors and Temperature on Oxygen Binding Properties and the Bohr Effect of Bovine Hemoglobin(Physiology)

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概要

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• The O_2 binding properties of bovine Hb were examined. The increase in Cl^- and DPG concentration enhanced P_<50>. A reduction in n_<max> was observed at high Cl^- concentration, while DPG had little effect on n_<max>. An increase in Cl^- concentration enhanced the Bohr effect, the magnitude of which reached a maximum at 0.1M Cl^- and 20℃. This concentration is nearly equal to that at the highest slope of the log P_<50> vs. log [Cl^-] plot, and also equal to the physiological Cl^- concentration (0.1M) of bovine blood. Furthermore, the influence of Cl^- concentration on the Bohr effect is independent of temperature. On the other hand, in the absence of Cl^-, bovine Hb is sensitive to DPG; an increase in DPG concentration enhanced the Bohr effect, which reached a maximum at 3mM DPG and 20℃. This concentration is nearly equal to that at the highest slope of the log P_<50> vs. log [DPG] plot. At low DPG concentrations, the DPG effect on the Bohr effect became small with increasing temperature, whereas at high DPG concentrations, the DPG effect was insensitive to temperature changes. At the physiological concentration of DPG (0.5mM), increases in both Cl^- concentration and temperature diminished the DPG effect. At the physiological concentrations of Cl^- and DPG, the Bohr effect was -0.36 at 37℃. The ΔH value at the physiological concentrations of Cl^- and DPG was approximately -5.8 kcal/mol at pH 7.4. These results indicate that Cl^- and temperature are important determinants of the O_2 binding properties of bovine Hb.

• 社団法人日本動物学会の論文
• 2006-06-25

著者

• Imai K

  Deparment Of Material Chemistry Faculty Of Engineering Hosei University

• Imai Kiyohiro

  Department Of Physiology And Biosignaling Graduate School Of Medicine Osaka University

• Kobayashi M

  Department Of Biology Faculty Of Science Niigata University

• Kobayashi Michiyori

  Department of Biology, Faculty of Science, Niigata University

• Sasagawa Keisuke

  Graduate School of Science and Technology, Niigata University

• Kobayashi Makito

  Department Of Aquatic Bioscience Graduate School Of Agricultural And Life Sciences The University Of

• Imai Kiyohiro

  Deparment Of Material Chemistry Faculty Of Engineering Hosei University

• Kobayashi M

  Epma Laboratory Niigata University Center For Instrumental Analysis

• Ishigaki K

  Deparment Of Material Chemistry Faculty Of Engineering Hosei University

• Sasagawa Keisuke

  Graduate School Of Science And Technology Niigata University

• Kobayashi M

  Ishigaki Tropical Station Seikai National Fisheries Research Institute

• Kobayashi Michiyori

  Department Of Biology Faculty Of Science Niigata University

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