Hemoglobins from the Two Closely Related Clams Barbatia lima and Barbatia virescenes. Comparison of Their Subunit Structures and N-terminal Sequence of the Unusual Two-Domain Chain : Biochemistry
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概要
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The intracellular hemoglobins were isolated from the two closely related arcid clams Barbatia lima and Barbatia virescens, respectively, and their subunit structures were compared in detail. B. virescens hemoglobin on Ultrogel AcA44 column had a single peak corresponding to the molecular weight (Mr) of 30,000. The hemoglobin was separated by high-performance liquid chromatography (HPLC) into two chains (I and II) with Mr 16,000, suggesting the heterodimeric structure. N-Terminal 20 residues of chain I showed homology (35% identity) with those of Anadara homodimeric hemoglobins. On the other hand, B. lima hemoglobin on Ultrogel AcA44 column had two peaks, a polymeric hemoglobin eluted in the void volume of the column and a Mr 60,000 hemoglobin. The polymeric hemoglobin on HPLC eluted as a single peak, and the Mr was estimated to be 32,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of a reducing agent. Therefore, it was suggested that the constituent chain of polymeric hemoglobin has an unusual two-domain structure with two hemes per chain. The Mr 60,000 hemoglobin was separated by HPLC into two major chains (A and B) with Mr 16,000 suggesting the tetrameric structure (A_2 B_2). These structural features of B, lima hemoglobins were similar to those of B. reeveana [1]. N-Terminal sequences of 36 residues of each domain of B. lima two-domain chain were determined from three CNBr fragments. N-Terminus of the chain was blocked by an acetyl group, and the second domain appeared to start at methionine residue. The sequence homology between domains were very high; 27 out of 36 residues (75%) were identical. We propose from these results that the two-domain chain resulted from gene duplication and the following loss of a stop codon.
- 社団法人日本動物学会の論文
- 1989-04-15
著者
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Suzuki Tomohiko
Department of Biology, Faculty of Science, Kochi University
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FURUKOHRI TAKAHIRO
Department of Biology, Faculty of Science, Kochi University
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Suzuki Tomohiko
Department Of Biology Faculty Of Science Kochi University
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Kobayashi Michiyori
Department Of Biology Faculty Of Science Niigata University
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SHIBA MAKOTO
Department of Biology, Faculty of Science, Kochi University
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Furukohri T
Kochi Univ. Kochi
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Furukohri Takahiro
Department Of Biology Faculty Of Science Kochi University
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Shiba Makoto
Department Of Biology Faculty Of Science Kochi University
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