The Deep-Sea Tube Worm Hemoglobin : Subunit Structure and Phylogenetic Relationship with Annelid Hemoglobin : Biochemistry

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概要

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• The deep-sea giant tube worm Lamellibrachia (the phylum Vestimentifera) contains two extracellular hemoglobins, a 3,000 kDa hemoglobin consisting of six chains (AI-VI) and a 440 kDa hemoglobin consisting of four chains (BI-IV) (Suzuki et. al., 1988, Biochem. J., 255, 541-545). The subunit structures of the hemoglobins were investigated by polyacrylamide gel electrophoresis (PAGE). In sodium dodecyl sulfate (SDS), the unreduced 440 kDa hemoglobin dissociated into three subunits; two "myoglobin-like" monomers (BIII and BIV) and a disulfide-bonded dimer of chains BI and BII, while the 3,000 kDa hemoglobin dissociated into five subunits; two monomers (AIII and AIV), a disulfide-bonded dimer of chains AI and AII, and two 32-36 kDa linker subunits (AV and AVI). The dissociation pattern of 3,000 kDa hemoglobin resembles that of leech giant hemoglobin. A gel filtration study on the hemoglobins exposed to alkaline pH or 4 M urea showed that Lamellibrachia 3,000 kDa hemoglobin is much more susceptible to dissociation than 440 kDa hemoglobin. Furthermore, the 3,000 kDa hemoglobin is autoxidized about ten times faster than 440 kDa hemoglobin. These results suggest that the subunit assembly of 3,000 kDa hemoglobin is rather unstable. A molecular model, [(BI, BII, BIII)_2BIV]_4, for the subunit assembly of stable 440 kDa hemoglobin is proposed. Amino acid analyses of the isolated Lamellibrachia chains and structural comparison with annelid chains showed that chains AI, AII, AIII, BII and BIII have an additional free cysteine residue. This residue appears to be one of the most probable candidates for the sulfide binding site of the tube worm hemoglobin. Such a cysteine may be acquired by a molecular adaptation of hemoglobin, in order to transport sulfide to intemal sulfide-oxidizing bacteria. A phylogenetic tree was constructed from N-terminal partial sequences of 9 Lamellibrachia chains and 10 annelid chains. The tree showed that there are two distinct strains for the heme-containing globin chains of the phyla Vestimentifera and Annelida, consistent with our previous proposal that the vestimentiferan tube worms should be placed in the phylum Annelida (Suzuki et. al., 1988, Biochem. J. 255, 541-545).

• 社団法人日本動物学会の論文
• 1989-10-15

著者

• Takagi T

  Aisin Seiki Co. Ltd. Kariya‐shi Jpn

• Ohta Suguru

  Ocean Researcg Institute, University of Tokyo

• Suzuki T

  Department Of Urology Gunma Cancer Center

• Ohta S

  Univ. Tokyo Tokyo Jpn

• Okuda Kazuo

  Department Of Biology Faculty Of Science Kochi University

• TAKAGI Takashi

  Biological Institute, Graduate Shool of Science, Tohoku University

• Suzuki Tomohiko

  Department of Biology, Faculty of Science, Kochi University

• FURUKOHRI TAKAHIRO

  Department of Biology, Faculty of Science, Kochi University

• Suzuki Tomohiko

  Department Of Biology Faculty Of Science Kochi University

• Furukohri Takahiro

  Department Of Biology Faculty Of Science Kochi University

• Takagi Takashi

  Biological Institute Faculty Of Science Tohoku University

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