Characteristics of the Deposition of Microfibrils during Formation of the Polylamellate Walls in the Coenocytic Green Alga Chamaedoris orientalis

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Characteristics of the deposition of cellulose microfibrils during formation of polylamellate walls and the arrangement of cortical microtubules in the tip-growing bipolar cells of Chamaedoris orientalis were examined by replica preparation methods and indirect immunofluorescence microscopy. The polylamellate wall is made up of two or three kinds of wall lamella which differ in terms of the orientation of microfibrils. Individual lamellae were periodically initiated one after another from the pole that was situated exactly at each growing apex of the cell and they were deposited basipetally. The orientation of microfibrils in each lamella was constant during deposition. Microfibrils in different lamellae were deposited at the same time through the cell wall but the timing of the deposition was staggered between neighboring lamellae so that the microfibrils would not be interwoven. By contrast, cortical microtubules were persistently arranged longitudinally all over the cell and no focal points to which they converged helically were visible, even around the cell apices. The mechanisms that regulate the formation of the polylamellate wall are discussed and a model for interpreting the involvement of the cortical microtubules in such mechanisms is proposed.
日本植物生理学会の論文