Viscous material production by clinical isolates of Prevotella intermedia under ethanol and salt stress

概要

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We have previously reported that some clinical isolates of Prevotella intermedia (P. intermedia) produced large amounts of extracellular viscous material that consisted mainly of neutral sugars, and that the capacity to produce exoplysaccharide (EPS) might allow this organism to live as a matrix-encased microbial community, rendering it highly resistant to unfavorable environmental conditions. In this study, we investigated how these clinical isolates (P. intermedia strains OD 1-2 and OD 1-16) change their productivity of EPS under ethanol and salt stress, which are known activators of biofilm formation. When trypticase soy broth (TSB) was supplemented with 3% ethanol (v/v), viscosities of spent culture media from OD 1-2 were significantly increased, although they were slightly decreased from OD 1-16. Supplementation of TSB with 1% (w/v) NaCI significantly enhanced the viscous material production on both OD 1-2 and OD 1-16. These results may suggest that P. intermedia has the capacity to augment its viscous material productivity under a stressed condition and that the response against ethanol stress is not identical among strains.