Effects of prototype surface reaction-type pre-reacted glass ionomer filler-containing root canal sealer on human periodontal ligament cells : mRNA expression of proinflammatory cytokines, osteoprotegerin and receptor activator of NF-kB ligand

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A prototype surface reaction-type pre-reacted glass ionomer filler-containing root canal sealer (FS) was developed for its potential to strengthen teeth and to inhibit microbial propogation by releasing multielements including fluoride. We investigated the direct effect of FS on human periodontal ligament cells (HPLCs) and compared its effect to that of the zinc oxide eugenol Endosealer^[○!R] (ES) by quantifying the gene expression responsible for inflammation and bone resorption. We used real-time reverse-transcliptase polymerase chain reaction to quantify the mRNA expression of IL-1β, IL-6, TNF-α, osteoprote-gerin(OPG) and receptor activator NF-kB ligand (RANKL) in HPLC in contact with FS. TNF-α mRNA expression induced by FS was significantly greater immediately after setting (FS0) and at 4 hours after setting (FS4). The mRNA expression of TNF-α and IL-6 induced by ES increased significantly at 4 hours after setting (ES4). Although the prototype S-PRG filler-containing root canal sealer (FS) showed a mild direct proinflammatory property, it did not directly affect bone resorption.