Effects of calcium hydroxide on expression of genes associated with hard tissue resorption in human osteoblastic cells stimulated by rhIL-1β
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概要
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We previously studied the effect of calcium hydroxide on the LPS-induced expression of hard tissue resorption-related genes in human osteoblastic cells (Saos-2), and reported that LPS-induced inflammatory cytokine expression and that the constant expression of RANKL was inhibited by calcium hydroxide. The objective of this study was to evaluate the effect of calcium hydroxide on the IL-1-induced expression of hard tissue resorption-related genes as a part of evaluation of the efficacy of calcium hydroxide for inflammatory hard tissue resorption. We used real-time reverse transcriptase-polymerase chain reaction to evaluate the effect of calcium hydroxide on the expression of genes of TNF-α, IL-6, OPG and RANKL synthesized by rhIL-1β-stimulated human osteoblast-like cells. The data were compared employing Student's t-test (n=6) with p<0.05 considered significant. Calcium hydroxide inhibited TNF-α and IL-6 gene expression in rhIL-1β-stimulated Saos-2, inhibited the enhancement of RANKL gene expression, and inhibited constant RANKL gene expression. Calcium hydroxide appeared to both resolve non-bacterial inflammation and inhibit hard tissue resorption.
著者
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YOSHIDA Masahiro
Department of Applied Chemistry and Chemical Engineering
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Baba Tadahiko
Department of Endodontics Osaka Dental University
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Hayashi Hiroyuki
Department of Endodontics, Osaka Dental University
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Shida Muneyasu
Department of Endodontics, Osaka Dental University
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Taguchi Yoichiro
Department of Periodontology, Osaka Dental University
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Ueda Masatoshi
Department of Periodontology, Osaka Dental University
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Ueda Masatoshi
大阪歯科大学 細菌学
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Ueda Masatoshi
Department Of Periodontology Osaka Dental University
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Ueda Masatoshi
大阪歯科大学 歯研究 薬理
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Kon'i Hirotaka
大阪歯科大学 欠損歯列補綴咬合学
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Kon'i Hirotaka
Department Of Postgraduate Clinical Training Osaka Dental University
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Ueda Masatoshi
Osaka Dental Univ. Osaka Jpn
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Ueda Mika
Department Of Periodontology Osaka Dental University
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Shida Muneyasu
Department Of Endodontics Osaka Dental University
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Hayashi Hiroyuki
Osaka Dental Univ. Osaka Jpn
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Taguchi Yoichiro
Department Of Periodontology Osaka Dental University
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Hayashi Hiroyuki
Department Of Endodontics Osaka Dental University
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Mikami Yoshimasa
Department Of Endodontics Osaka Dental University
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Hayashi Hiroyuki
Department Of Cardiology Kanazawa Medical University
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Hayashi Hiroyuki
Department Of Applied Chemistry Faculty Of Engineering Osaka Institute Of Technology
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Yoshida Masahiro
Department Of Applied Bioorganic Chemistry Gifu University
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HAYASHI Hiroyuki
Department of Advanced Energy, University of Tokyo, Kashiwa, Chiba 277-8561, Japan
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