Intracellular pH Regulation in an Acidophilic Unicellular Alga, Cyanidium caldarium : ^<31>P-NMR Determination of Intracellular pH

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概要

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• The intracellular pH of an acidophilic unicellular alga, Cyanidium caldarium, was determined as a function of external pH by ^<31>P nuclear magnetic resonance. The algal cells incubated under aerobic conditions or under anaerobic and illuminated conditions maintained the intracellular pH in the range from 6.8 to 7.0 even when the external pH was changed from 1.2 to 8.4. Under anaerobic and dark conditions, however, the intracellular pH acidified at the acidic pH region of the external medium. The acidified intracellular pH reversibly returned to neutral either on aeration or illumination. The results indicate that, in Cyanidium cells growing in extremely acidic environments, an active H^+ efflux (H^+ pump) which depends on metabolic activity (respiration or photosynthesis) is essential to maintain the intracellular pH at a constant physiological level against the passive H^+ leakage due to the steep pH gradient across the cell membrane.

• 日本植物生理学会の論文

著者

• KYOGOKU Yoshimasa

  Institute for Protein Research, Osaka University

• Akutsu Hideo

  Department Of Physical Chemistry Faculty Of Engineering Yokohama National University

• Akutsu Hideo

  Department Of Bioengineering Faculty Of Engineering Yokohama National University

• Kyogoku Y

  Osaka Univ. Osaka

• Kyogoku Yoshimasa

  Institute For Protein Research Osaka University

• Enami Isao

  Department of Biology, Faculty of Science, Science University of Tokyo

• Enami Isao

  Department Of Biology Faculty Of Science Science University Of Tokyo

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