Respiration-Dependent H^+ Efflux from Intact Cells of Cyanidium caldarium
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概要
- 論文の詳細を見る
An active H^+ efflux depending on respiration was found in an acidophilic unicellular alga, Cyanidium caldariulm. Alkalization of the medium due to passive H^+ transport into the cells was observed when the respiratory activity was inhibited by adding respiratory poisons, such as rotenone or antimycin A, or by introducing pure nitrogen into the cell suspension. The extent of the H^+ influx increased as the pH of the medium was lowered to 2.9, indicating that H^+ Ieaks into the cells according to the pH gradient across the plasma membrane. The medium pH which had increased under anaerobic condition returned to the original level with aeration of the cell suspension. This suggests that an active H^+ transport, related to respiration, pumps out the excess H^ accumulated in the cells during anaerobic preincubation. The pH changes in the cell suspension were related to the intracellular ATP level. From these results it was concluded that active H^+ efflux dependent upon oxidative phosphorylation fLmctions in the dark to maintain a constant intracellular pH against passive H^+ leakage through the plasma membrane. The light-induced H^+ efflux and the respiration-dependent H^+ efflux were also compared in relation to the physiological role of the active H^+ efflnux, especially with respect to the intracellular pH regulation in this alga.
- 日本植物生理学会の論文
著者
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Enami Isao
Department Of Biology Faculty Of Science Science University Of Tokyo
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Kura-hotta Mariko
Department Of Biology Faculty Of Science Science University Of Tokyo:(present)department Of Pure And
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Kura-hotta Mariko
Department Of Biology Faculty Of Science Science University Of Tokyo
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