Light-induced H^+ Efflux from Intact Cells of Cyanidium caldarium
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概要
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Light-induced pH changes in suspensions of an acidophilic unicellular alga, Cyanidium caldarium Geitler, were studied as a function of the pH of the medium. In the neutral pH region, alkalization of the medium due to photosynthetic CO_2 uptake was observed. In the acidic pH region, illumination caused a significant decrease in the pH of the medium, indicating the efflux of H^+ from the cells. Both the rate and extent of the pH decrease increased as the pH of the medium was lowered to 3.0. The H^+ efflux was not affected by 3-(3',4'-dichlorophenyl)-1,1-dimethylurea, but was inhibited by phenyl-mercuric acetate. The fastest H^+ efflux occurred at 45℃, whereas its extent was almost constant from 25 to 50℃. The activity decreased at temperatures above 50℃ and was inactivated completely at 60℃. Its action spectrum corresponded the spectrum for chlorophyll a absorption. Results indicate that the light-induced H^+ efflux is driven by photosystem I and is important in the maintenance of the intracellular pH at the functional neutral region against a steep pH gradient across the cell membrane.
- 日本植物生理学会の論文
著者
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Enami Isao
Department Of Biology Faculty Of Science Science University Of Tokyo
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Kura-hotta Mariko
Department Of Biology Faculty Of Science Science University Of Tokyo
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