Mechanisms of the acido-and thermophily of Cyanidium caldarium Geitler III. Loss of these characteristics due to detergent treatment
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概要
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The photochemical reactions of Cyanidium cells treated with various concentrations of Triton X-100 or digitonin were examined. As the concentration of Triton X-100 was increased, the following 4 responses were observed : inhibition of endogenous 02 evolution (above 0.005%), stimulation of the p-BQ Hill reaction (above 0.01%), loss of thermophily (above 0.03%) and loss of acidophily (above 0.5%). In the presence of Triton X-100 (0.1% of final concentration), the p-BQ Hill reaction showed optimum activity at 30℃ and was completely inactivated at temperatures over 45℃, though the optimum activity m the absence of Triton X-100 was 45℃. The pH activity curve, however, was unchanged by treatment with Triton X-100. The loss of heat tolerance caused by Triton X-100 was observed not only in the Hill reaction but also in the photo-system I reaction. The thermophily of Triton X-100-treated cells was completely recovered after washing with distilled water. The acidophily of the alga was lost after digitonin (0.01% of final concentration) treatment without any loss of thermophily or the inactivation of photosystem I and II reactions. The p-BQ Hill activity of digitonin-treated cells was optimum at pH 7 and completely lost in acid pH regions, while the temperature dependency was unchanged by this treatment. The irreversible loss of acid tolerance of the photosystem I and II activities due to digitonin was confirmed by various acid and alkaline treatments.
- 日本植物生理学会の論文
著者
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Enami Isao
Department Of Biology Faculty Of Science Science University Of Tokyo
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Fukuda Ikujiro
Department Of Biology Faculty Of Science Science University Of Tokyo
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