Mechanisms of the acido- and thermo-phily of Cyanidium caldarium Geitler II. Physiological role of the cell wall
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概要
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1) A partially disintegrated cell preparation of Cyanidium caldarium (i.e. the 100G faction; cells from which the outer parts of the cell wall had been removed) was obtained by differential centrifugation of a cell suspension treated with a French press. 2) The 100G fraction cells had completely lost the outer parts of their cell walls, but retained their subcellular structure unchanged and had Hill activity that was equally as high as that of the intact cells. 3) The Hill activity of the 100G fraction showed an optimum at pH 7.0 and at 35℃. This activity was lost under acid and high temperature conditions (e.g. pH 3, 50℃) under which intact cells showed high activity. 4) The Hill activity of the 100G fraction was lost by heat treatment (55℃, 10 min), acid-treatment (pH 3.0, 10 min) or pre-illumination (3 × 10^5 lux, 30 min) though in-tact cells were not inactivated by these treatments. However, no remarkable difference in sensitivity towards inhibitors was found between the 100G fraction and intact cells. 5. We thus concluded that the cell wall plays an important role in the acido- and thermo-phily of Cyanidium cells.
- 日本植物生理学会の論文
著者
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Enami Isao
Department Of Biology Faculty Of Science Science University Of Tokyo
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Fukuda Ikujiro
Department Of Biology Faculty Of Science Science University Of Tokyo
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Nagashima Hideyuki
Department Of Biology Faculty Of Science Science University Of Tokyo
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Nagashima Hideyuki
Department Of Biological Science And Technology Sciecnce Univeristy Of Tokyo
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