Stable Isotope Labeled Cytochrome c_3 from Desulfovibrio vulgaris on a Defined Medium as Sole Nitrogen Source

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概要

• 論文の詳細を見る

• The conditions of cultivation and the composition of medium for Desulfovibrio vulgaris Miyazaki F (DvMF) were examined to obtain cytochrome c_3 labeled with a stable isotope. The growth of DvMF was steady and reproducible under purging with N_2 and under pH control. DvMF was able to grow on a defined medium without natural products. The composition of medium containing a small amount of NH_4Cl as sole nitrogen source was established. Then, [U-^<15>N]cytochrome c_3 was obtained during the culture of DvMF in a defined medium with ^<15>NH_4Cl; it was confirmed by ^1H-^<15>N HMQC. This is the first report of [U-^<15>N]cytochrome c_3.

• 社団法人日本農芸化学会の論文
• 1996-12-23

著者

• Akutsu H

  Yokohama National Univ. Yokohama Jpn

• Akutsu Hideo

  Department Of Bioengineering Faculty Of Engineering Yokohama National University

• OHMURA Tomoaki

  Biotechnology Laboratory, Advanced Technology Research Center, Mitsubishi Heavy Industries, Ltd.

• NAKAMURA Takeshi

  Biotechnology Laboratory, Advanced Technology Research Center, Mitsubishi Heavy Industries, Ltd.

• Ohmura Tomoaki

  Biotechnology Laboratory Advanced Technology Research Center Mitsubishi Heavy Industries Ltd.

• Nakamura Takeshi

  Biotechnology Laboratory Advanced Technology Research Center Mitsubishi Heavy Industries Ltd.

関連論文

• Intracellular pH Regulation in an Acidophilic Unicellular Alga, Cyanidium caldarium : ^P-NMR Determination of Intracellular pH
• Cloning and Expression of the Gene Encoding Flavodoxin from Desulfovibrio vulgaris(Miyazaki F)^1
• Purification and Characterization of Homo- and Hetero-Dimeric Acetate Kinases from the Sulfate-Reducing Bacterium Desulfovibrio vulgaris
• A Gene Encoding a Cytochrome c Oxidase-Like Protein Is Located Closely to the Cytochrome c-553 Gene in the Anaerobic Bacterium, Desulfovibrio vulgaris (Miyazaki F)
• Osmotic Stability of Muramyl Dipeptide-Bearing Liposomes and Molecular Miscibility in Their Membranes
• A Simple, Rapid, and Highly Efficient Gene Expression System for Multiheme Cytochromes c
• Stable Isotope Labeled Cytochrome c_3 from Desulfovibrio vulgaris on a Defined Medium as Sole Nitrogen Source

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