A Simple, Rapid, and Highly Efficient Gene Expression System for Multiheme Cytochromes c
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概要
- 論文の詳細を見る
The genes of tetraheme cytochrome c_3 and hexadecaheme high-molecular-weight cytochrome c from Desulfovibrio vulgaris could be overexpressed as holoproteins in Shewanella oneidensis TSP-C using pUC-type vectors of E. coli. Surprisingly, S. oneidensis was transformed directly by pUC-type vectors through electroporation. The yields of the recombinant proteins in this expression system were much higher than the previously reported ones.
- 社団法人日本農芸化学会の論文
- 2001-01-23
著者
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Akutsu Hideo
Department Of Chemistry And Biotechnology Faculty Of Engineering Yokohama National University:instit
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Akutsu Hideo
Department Of Bioengineering Faculty Of Engineering Yokohama National University
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Ozawa Kiyoshi
Department Of Bioengineering Faculty Of Engineering Yokohama National University
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Fujiwara Yumiko
Department Of Chemistry And Biotechnology Faculty Of Engineering Yokohama National University
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Yasukawa Fumiko
Department Of Chemistry And Biotechnology Faculty Of Engineering Yokohama National University
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Ozawa Kiyoshi
Department of Biochemistry and Molecular Biology, Faculty of Medicine, Nippon Medical School
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