Preformed mRNA and Light-induced Germination of Pine (Pinus thunbergii) Seed

概要

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The mRNAs were isolated from dry, dark-imbibed and light-imbibed pine (Pinus thunbergii) seeds, in which germination is induced upon exposure to light, and their translational activities in a wheat embryo cell-free system were examined. A portion of the mRNA extracted from each type of seed appeared to be poly(A)+RNA. A significant translational activity was already present in the RNA fraction isolated from the dry pine seeds. The preformed mRNA seems not to be translated in vivo during the dark-imbibition, since most of the in vivo protein synthesis did not occur until the seeds were exposed to light. The SDS polyacrylamide gel electrophoregrams of the polypeptides synthesized in vitro in response to either the preformed mRNA or the mRNAs from the dark-imbibed or light-imbibed seeds were qualitatively identical; thus it seems that the preformed mRNA is conserved during dark imbibition, and that its translation is initiated after the exposure of the imbibed seeds to light.
日本植物生理学会の論文
1982-07-00