Characteristics of Isolated Microsporocytes from Liliaceous Plants for Studies of the Meiotic Cell Cycle in Vitro

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概要

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• Experiments were aimed at obtaining a culture system for study of the differentiation of meiotic cells, and techniques were successfully established for the in vitro culture of premeiotic microsporocytes isolated from various species of liliaceous plants. The problems concerning the culture system were whether microsporocytes could be isolated at each stage of the premeiotic interphase and whether they would survive and undergo cell division at high frequencies in culture media. In the species tested, the range of stages permitting isolation from anthers and the cytological features of the extruded cells were examined in some detail. Culture conditions are defined for the survival and the induction of cell division in explanted cells, and some factors influencing these frequencies are discussed. Explanted cells at premeiotic G_1 and S phases underwent a mitotic division, whereas once cells entered the leptotene stage they completed meiosis. Cells explanted at the G_2 phase showed abnormal types of meiotic division. The culture system developed makes possible cytological, physiological and biochemical analyses of premeiotic cells cultured under various conditions.

• 日本植物生理学会の論文

著者

• ITO Michio

  Department of Dental Materials, Matsumoto Dental University

• YOSHIOKA Masaharu

  Department of Biology, Nagoya University

• Yoshioka Masaharu

  Department Of Biology. Faculty Of Science Nagoya University

• Takegami Miyako

  Department of Biology. Faculty of Science, Nagoya University

• Tanaka Ichiro

  Department of Biology. Faculty of Science, Nagoya University

• Takegami Miyako

  Department Of Biology Faculty Of Science Nagoya University

• Takegami Miyako

  Department Of Biology. Faculty Of Science Nagoya University

• Tanaka Ichiro

  Department Of Biology Faculty Of Science Nagoya University

• Ito Michio

  Department of Biology, Faculty of Science, Nagoya University

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