Studies on the control of cell division in a green alga, Ankistrodesmus gracilis (Chlorococcales) III. Induction synchrony with controlled division patterns

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When stagnant cells of Ankistrodesmus gracilis obtained from a standard culture were inoculated into the basal medium at cell densities lower than 1.0×10^7 cells/ml, cell proliferation occurred stepwise at time intervals of about 30 hr. At a density of 5.5×10^4 cells/ml, the increase in cell number per step was about 2.7-fold. When inoculated into a glutamine medium the time interval was 24 hr, and the average increase of cell number was about 4-fold. When cells were preincubated at about 5.0×10^5 cells per ml in the basal medium for 30 hr, then transferred into a glutamine or arginine medium at about 7.0×10^6 cells/ml, synchronous division occurred about 18 hr later with binary fission or about 33 hr later with multiple fission, respectively.
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