Time-Dependent Fluorescence Depolarization of Fluorescein in Rat Thymus Lymphocytes
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概要
- 論文の詳細を見る
Time behavior of fluorescence depolarization has been measured for fluoresceinmolecules introduced into rat thymus lymphocytes by means of a time-correlatedsingle-photon-counting method under the excitation by a CW mode-locked Ar" ionBaser. The depolarization mechanism has been identified as restricted rotationalBrownian motion of fluorescein in the case of very dilute dye concentration. Theobserved temperature dependence of rotatable angle of fluorescein as well as that ofrotational relaxation time is consistent with the expectation that the lymphocytebecomes softer as the temperature is raised. The relation between the quantitiesobtained by stationary and time-resolved experiments is discussed.
- 社団法人日本物理学会の論文
- 1982-02-15
著者
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Tanaka Ichiro
Department of Biology. Faculty of Science, Nagoya University
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Kinoshita Shuichi
Department Of Electrical Engineering National Defense Academy
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Kimura Shuhei
Department Of Physiology Osaka City University Medical School
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Kinoshita Yoshihiro
Department Of Gastroenterological Surgery Toranomon Hospital
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Kinoshita Yoshihiro
Department Of Physiology Osaka City University Medical School
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Kinoshita Yoshihiro
Department Of Aggressology And Critical Care Medicine Faculty Of Medical And Pharmaceutical Sciences
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Tanaka Ichiro
Department Of Biology Faculty Of Science Nagoya University
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Tanaka Ichiro
Department Of Physics Osaka University
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Kinoshita Shuichi
Department of Chemistry, Faculty of Science, University of Tokyo
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