TiプラスミドによるNicotiana glauca形質転換細胞における,アミノグリコシド,フォスフォトランスフェレ-ス2,ノパリン,シンテ-ス転写産物の解析〔英文〕

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概要

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• The vector possessing two marker genes encoding for, aminoglycoside phosphotransferase II (APH(3′)II) and nopaline synthase (NOS) was inserted into the octopine-type Ti plasmid through homologous recombination. Following the confirmation of foreign gene expression in Nicotiana glauca tumor cells with respect to kanamycin resistance and nopaline synthesis, the analysis was made on the transcripts. There was a quantitative difference in the amount of mRNA between APH(3′)II and NOS. The size of mRNA for APH(3′)II and NOS were 1250 bases and 1550 bases, respectively.

• 日本遺伝学会の論文

著者

• 小野 道之

  Institute of Biological Sciences, University of Tsukuba

• HASHIMOTO Hiroyuki

  Institute of Biological Sciences, University of Tsukuba

• YOSHIOKA Masaharu

  Department of Biology, Nagoya University

• HARADA Hiroshi

  Institute of Biological Sciences, University of Tsukuba

• UCHIMIYA Hirofumi

  Institute of Biological Sciences, University of Tsukuba

• Uchimiya Hirofumi

  Institute For Molecular And Cellular Biosciences University Of Tokyo

• Hashimoto Hiroyuki

  Institute High Speed Mechanics Tokyo University

• Harada Hiroshi

  Institute Of Biolo Gical Sciences The University Of Tsukuba

• ONO Michiyuki

  Institute of Biological Sciences, University of Tsukuba

• 橋本 博之

  Institute of Biological Sciences, University of Tsukuba

• 吉岡 正陽

  Department of Biology, Nagoya University

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